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Chapter 1: Human iPSC colony morphology

Morphology of human iPS cell colonies is important to judge the condition of the culture. Colonies in a good condition show high cell density inside.

Chapter 2: Seeding feeder cells

The plating density of feeder cells is important for human iPS cell culture.

Chapter 3: Passaging human iPS cells


Dissociation Solution for human ES/iPS Cells (CTK solution)

makes it possible to passage human iPS cells very easily and successfully with excellent cell viability. Colonies can be divided to appropriate size for passaging just by incubation and pipetting.


Chapter 4: Freezing and thawing human iPS cells

It is possible to cryopreserve human iPS cells at high cell viability by using Freezing Medium for human ES/iPS Cells. This method ulitizes vitrification which minimizes the damage to the cryopreserved cells. It is important to freeze and thaw the cells quickly.

Chapter 5: Transition from feeder-dependent culture to feeder-free culture

Transition from feeder-dependent culture to feeder-free culture is easy with ReproCELL’s new feeder-free medium ReproFF2. No special adaptation procedure is required. Laminin-5 and Matrigel can be used as coating material.

Chapter 6: Passaging procedure for feeder-free culture

Feeder-free culture with ReproFF2 is easy. Passaging and cryopreservation procedures are the same as those for feeder-dependent culture.