Dependent tumor angiogenesis requires inverse and reciprocal regulation of VEGFR1 and VEGFR2

Z Zhang, K G Neiva, M W Lingen, L M Ellis and J E Nör
Edited by G Rabinovich

Cell Death and Differentiation (2010) 17, 499–512; doi:10.1038/cdd.2009.152;
published online 16 October 2009

The glycosylation status of VEGFR2 was evaluated with the Enzymatic CarboRelease kit (QA-Bio, Palm Desert, CA, USA), according to the manufacturer’s instructions. HDMECs
were starved overnight and treated with 50 ng/ml VEGF for 20 min. …