DEPArray™ NxT 單細胞分離篩選系統

DEParray^™ 在腫瘤學Oncology的應用

• Mangano et al, Blood Cancer Journal 2019, ” Precise detection of genomic imbalances at single-cell resolution reveals intra-patient heterogeneity in Hodgkin’s lymphoma“
• D’Oronzo et al, Scientific Reports 2019, ” Dissection of major cancer gene variants in subsets of circulating tumor cells in advanced breast cancer“
• Chemi et al, Nature Medicine 2019, ” Pulmonary venous circulating tumor cell dissemination before tumor resection and disease relapse“
• Jang Ho Cho et al, Translational Cancer Research 2019, ” Detection of circulating tumor cells (CTCs) in cerebrospinal fluid of a patient with HER2-overexpressing gastric cancer and single cell analysis of intra-patient heterogeneity of CTCs“
• Brouwer at al, PLOS ONE 2019, ” HER-2 status of circulating tumor cells in a metastatic breast cancer cohort: A comparative study on characterization techniques“
• Pailler et al, Clinical Cancer Research 2019, ” Acquired Resistance Mutations to ALK-Inhibitors Identified by Single Circulating Tumor Cell Sequencing in ALK-Rearranged Non-Small-Cell Lung Cancer“
• Wu et al, Int Journal of Molecular Sciences 2019, ” Nuclear-Biased DUSP6 Expression is Associated with Cancer Spreading Including Brain Metastasis in Triple-Negative Breast Cancer“
• Cariati et al, Int Journal of Molecular Sciences 2019, ” Dissecting Intra-Tumor Heterogeneity by the Analysis of Copy Number Variations in Single Cells: The Neuroblastoma Case Study“
• Meyer et al, Human Pathology 2019 ” Co-expression of cytokeratin and vimentin in colorectal Cancer highlights a subset of tumor buds and an atypical Cancer-associated stroma“
• Hodara et al, Journal od Clinical Oncology Insights 2019, ” Multi-parametric liquid biopsy analysis in metastatic prostate cancer“
• Dorssers et al, British Journal of Cancer 2019, ” Molecular heterogeneity and early metastatic clone selection in testicular germ cell cancer development“
• Rihawi et al, Translational Oncology 2019, ” Amplification as a Potential Mechanism of Primary Resistance to Crizotinib in ALK-Rearranged Non-Small Cell Lung Cancer: A Brief Report“
• Vishnoi et al, Cancer Research 2018, ” Targeting USP7 Identifies a MetastasisCompetent State within Bone Marrow–Resident Melanoma CTCs“
• De Laere et al, Clinical Cancer Research 2018, ” TP53 outperforms other androgen receptor biomarkers to predict abiraterone or enzalutamide outcome in metastatic castration-resistant prostate cancer“
• Chalopin et al, Journal of Bone Oncology, 2018 ” Isolation of circulating tumor cells in a preclinical model of osteosarcoma: effect of chemotherapy“
• Petrossian et al, Oncotarget 2018, ” ERα-mediated cell cycle progression is an important requisite for CDK4/6 inhibitor response in HR+ breast cancer“
• Ji Won Lee et al, Journal of Applied Genetics 2018, “Identification of novel mutations in FFPE lung adenocarcinomas using DEPArray sorting technology and next-generation sequencing”
• Ferrarini A et al, PLOS ONE 2018, “A streamlined workflow for single-cells genome-wide copy-number profiling by low-pass sequencing of LM-PCR whole-genome amplification products”
• Boulding et al, Scientific Reports 2018, ” LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer“
• Paoletti et al, Cancer Research 2017, ” Comprehensive mutation and copy number profiling in archived circulating breast cancer tumor cells documents heterogeneous resistance mechanisms“
• Boral et al, Nature Communications 2017, “Molecular characterization of breast cancer CTCs associated with brain metastasis”
• Mesquita et al, Molecular Oncology 2017, “Molecular analysis of single circulating tumour cells following long-term storage of clinical samples”
• Paolillo et al, Clinical Cancer Research 2017, ” Detection of Activating Estrogen Receptor Gene (ESR1) Mutations in Single Circulating Tumor Cells“
• Palmirotta et al, Cancer Genomics and Proteomics 2017, “Next-generation Sequencing (NGS) Analysis on Single Circulating Tumor Cells (CTCs) with No Need of Whole-genome Amplification (WGA)”
• Rapp C et al, Acta Neuropathol 2017, ““Identification of T cell target antigens in glioblastoma stem‑like cells using an integrated proteomics‑based approach in patient specimens”
• Bingham C et al, Breast Cancer Res Treat 2017, “Mutational studies on single circulating tumor clles isolated form the blood of inflammatory breast cancer patients”
• Tellez Gabriel M et al, European Journal of Cell Biology 2017, “Analysis of gap junctional intercellular communications using a dielectrophoresis-based microchip”
• De Laere B et al, JTS 2016, “Patients with metastatic hormone receptor-positive breast cancer express PIK3CA oncogene mutational heterogeneity in circulating tumor cells”
• Carter L et al, Nature Medicine 2016, “Molecular analysis of circulating tumor cells identifies distinct copy-number profiles in patients with chemo sensitive and chemo refractory small-cell lung cancer”
• Zhaomei Mu et al, IJMC 2016, “Detection and Characterization of Circulating Tumor Associated Cells in Metastatic Breast Cancer”
• Shaw J et al, Clinical Cancer Research 2016, “Mutation analysis of cell-free DNA and single circulating tumor cells in metastatic breast cancer patients with high CTC counts”
• Millner LM et al, Cancer Research Frontiers 2016, “Comprehensive isolation, identification, and nucleic acid analysis of single breast cancer cells: CTC-isoTECH”
• De Luca F et al, Oncotarget 2016, “Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer”
• Bulfoni M et al, Breast Cancer Research 2016, “In patients with metastatic breast cancer the identification of circulating tumor cells in epithelial-to-mesenchymal transition is associated with a poor prognosis”
• Yee S.S et al, Molecular Genetics and Genomic Medicine 2016, “A novel approach for next-generation sequencing of circulating tumor cells”
• Bolognesi C et al, Nature Scientific Reports 2016, “Digital Sorting of Pure Cell Populations Enables Unambiguous Genetic Analysis of Heterogeneous Formalin-Fixed Paraffin-Embedded Tumors by Next Generation Sequencing”
• Rothwell G et al, Molecular Oncology 2015, “Genetic profiling of tumours using both circulating free DNA and circulating tumour cells isolated from the same preserved whole blood sample”
• Vishnoi M et al, Nature Scientific Reports 2015, “The isolation and characterization of CTC subsets related to breast cancer dormancy”
• Salvianti et al, Biomolecular Detection and Quantification 2015, ” Feasibility of a workflow for the molecular characterization of singlecells by next generation sequencing”
• Ewelina Krzywinska et al, EBioMedicine 2015, “Identification of Anti-tumor Cells Carrying Natural Killer (NK) Cell Antigens in Patients with Hematological Cancers”
• Maltoni R et al, Cancer Letters 2015, “Circulating tumor cells in early breast cancer: A connection with vascular invasion”
• Pestrin M et al, Molecular Oncology 2014, “Heterogeneity of PIK3CA mutational status at the single cell level in circulating tumor cells from metastatic breast cancer patients”
• Fernandez S.V. et al, Breast Cancer Research 2014, “TP53 mutations detected in circulating tumor cellspresent in the blood of metastatic triple negative breast cancer patients“
• Carpenter E, et al, Frontiers in Oncology 2014, “Dielectrophoretic capture and genetic analisys of single neuroblastoma tumor cells”
• Hodgkinson C.L.et al, Nature Medicine 2014, “Tumorigenicity and genetic profiling of Circulating Tumor Cells in Small Cell Lung Cancer”
• Peeters DJE, et al. British Journal of Cancer 2013, “Semiautomated isolation and molecular characterization of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting”
• Fabbri F, et al. Cancer Letters 2013, “Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs”

DEParray^™ 在法醫鑑識Forensic的應用

• Anslinger et al, FSI Genetics Supplement Series 2019, “New strategies in the field of mixture deconvolution single cell STR profiling“
• Anslinger et al, Rechtsmedizin 2019, “Deconvolution of blood-blood mixtures using DEPArrayTM separated single cell STR profiling“
• Anslinger K et al, International Journal of Legal Medicine 2018, “Whose blood is it? Application of DEPArray^™ technology for the identification of individual/s who contributed blood to a mixed stain“
• Williamson VR et al, FSI Genetics 2018, “Enhanced DNA Mixture Deconvolution of Sexual Offense Samples Using the DEPArray^TM System“
• Aslinger K et al, Rechtsmedizin 2017, “Application of DEPArray^™ technology for the isolation of white blood cells from cell mixtures in chimerism analysis”
• Fontana F et al, FSI Genetics 2017, “Isolation and genetic analysis of pure cells from forensic biological mixtures: The precision of a digital approach”
• Hansson O et al, FSI Genetics 2017, “Characterization of artefacts and drop-in events using STR-validator and single-cell analysis”

DEParray^™ 在蠟塊包埋FFPE的應用

• Meyer et al, Human Pathology 2019 “Co-expression of cytokeratin and vimentin in colorectal Cancer highlights a subset of tumor buds and an atypical Cancer-associated stroma“
• Dorssers et al, British Journal of Cancer 2019, “Molecular heterogeneity and early metastatic clone selection in testicular germ cell cancer development“
• Isidori et al, BMC Cancer 2018, “Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report“
• Ji Won Lee et al, Journal of Applied Genetics 2018, “Identification of novel mutations in FFPE lung adenocarcinomas using DEPArray sorting technology and next-generation sequencing”
• Bolognesi C et al, Nature Scientific Reports 2016, “Digital Sorting of Pure Cell Populations Enables Unambiguous Genetic Analysis of Heterogeneous Formalin-Fixed Paraffin-Embedded Tumors by Next Generation Sequencing”

DEParray^™ 在分離幹細胞Isolation of Stem Cells的應用

• Silvestris et al, Human Reproduction 2018, “In vitro differentiation of human oocytelike cells from oogonial stem cells: single-cell isolation and molecular characterization”

DEParray^™ 在蠟塊包埋FFPE應用的社論

• Kathy Liszewski, GEN 2016, “The Next Next Thing in Sequencing”
• Farideh Bischoff, Nicolò Manaresi and Chiara Bolognesi, GEN 2015, “Isolation of Pure Tumor Cell Populations”

DEParray^™技術出版品

以下參考文獻是有關於介電泳技術和移動細胞的原理

• Abonnenc M et al, J Immunol 2013, “Lysis-on-Chip of Single Target Cells following Forced Interaction with CTLs or NK Cells on a Dielectrophoresis-Based Array“
• Abonnenc M et al, Anal. Chem. 2013, “Programmable Interactions of Functionalized Single Bioparticles in a Dielectrophoresis-Based Microarray Chip“
• Gagnon ZR, Electrophoresis 2011, “Cellular dielectrophoresis: applications to the characterization, manipulation, separation and patterning of cells“
• Fabbri E et al, J Appl Polymer Sci 2008, “Levitation and movement of tripalmitin-based cationic lipospheres on a dielectrophoresis-based lab-on-a-chip device“
• Borgatti M et al, Int J Mol Med 2008, “New trends in non-invasive prenatal diagnosis: Applications of dielectrophoresis-based Lab-on-a-chip platforms to the identification and manipulation of rare cells“
• Vulto P et al, J Micromech Microeng 2006, “Selective sample recovery of DEP-separated cells and particles by phaseguide-controlled laminar flow“
• Fuchs AB et al, Lab Chip 2005, “Electronic Sorting and Recovery of Single Live Cells From Microlitre Sized Samples“
• Borgatti M et al, Int J Mol Med 2005, “Separation of White Blood Cells From Erythrocytes on a Dielectrophoresis (DEP) Based ‘Lab-On-A-Chip’ Device“
• Borgatti M et al, International Journal Oncology 2005, “Dielectrophoresis-based ‘Lab-on-a-chip’ devices for programmable binding of microspheres to target cells“
• Abonnenc M et al, NanoBiotechnology 2005, “A dielectrophoretic microchip for controlled cell targeting with functionalized microspheres“
• Medoro G et al, IEEE Sensors J 2003, “A lab-on-a-chip for cell detection and manipulation“
• Altomare L et al, Biotechnol and Bioeng 2003, “Levitation and movement of human tumor cells using a printed circuit board device based on software-controlled dielectrophoresis“
• Gascoyne PR et al, Electrophoresis 2002, “Particle separation by dielectrophoresis“
• Archer S et al, Biochem Biophys Res Commun 1999, “Cell Reactions to Dielectrophoretic Manipulation“

Brochure

• DEPArrayNxT_Brochure.pdf