ReproCELL Stemgent Self-Replicative RNA reprogramming (blood reprogramming) 重新編程試劑
Stemgent StemRNA-SR Reprogramming Kit Stemgent 致力於幹細胞研究，結合自我複製 RNA (self-replicative RNA) 和 microRNA 技術進行細胞編程，不論是周邊血或臍帶血皆可建立專屬的 iPS 細胞。無外來基因，無需使用病毒，無需餵養細胞 (Feeder-free)，從血液中分離出 EPCs 進行重新編程，只需兩次基因轉染 (transfection)。
分類：mRNA Reprogram > 幹細胞相關產品 > 試劑溶液
標籤：
最快，最安全之血液細胞重新編程為 iPS 細胞的方式
建立病人專屬 iPS 細胞
產品優勢
血液樣品 & 纖維細胞皆可產生高品質的人類 iPS 細胞
無病毒基因、無外來基因鑲嵌，安全性高，可臨床使用
優化實驗操作步驟，可獲得具重複性的結果
只需 2 次轉染，即可獲得大量 iPS 細胞
25 天之內即可獲得 iPS 細胞，且可成功分化。
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| BLOOD SOURCE | PRIMARY EPC ESTABLISHMENT EFFICIENCY | REPROGRAMMING EFFICIENCY (PATIENT TO PATIENT) |
|---|---|---|
| Peripheral Blood | 18/22 = 82% | 9/13 = 70% |
| Cord Blood | 46/53 = 87% | 33/41 = 81% |
| TOTAL | 64/75 = 85% | 42/54 = 78% |
表 1. 從人類血液樣品中分離出 EPC (Epithelial Progenitor Cells) 與重新編程的效率
根據完整實驗指引，在 6 孔盤大小，單孔可製備 10~20 株 iPS 細胞。
- (2013 )Yoshioka N; Gros E; Li H-R; Kumar S; Deacon DC; Maron C; Muortri AR; Chi NC; Fu X; Yu BD; Dowdy SF. “Efficient Generation of Human iPSCs by a Synthetic Self-Replicative RNA.” Cell Stem Cell 13:246
- (2012) Geti I; Ormiston M: Touhain F; Toshner M; Movassagh M; Nichols J; Mansfield W; Southwood M; Bradley A; Rana AA; Vallier L; Morrell NW. “A practical and efficient cellular substrate for the generation of induced pluripotent stem cells.” Stem Cells Trans med 1:855
- (2009) Yoshida Y; Takahashi K; Ishisaka T; Yamanaka S. “Hypoxia Enhances the Generation of Induced Pluripotent Stem Cells.” Cell Stem Cell 5:237
Kit & Reagent
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| 產品貨號 | 產品名稱 | 產品數量 | |
|---|---|---|---|
| 00-0075 | Stemgent StemRNA-SR Reprogramming Kit Content
| – |
- Yoshioka N; Gros E; Li H-R; Kumar S; Deacon DC; Maron C; Muortri AR; Chi NC; Fu X; Yu BD; Dowdy SF. Efficient Generation of Human iPSCs by a Synthetic Self-Replicative RNA. Cell Stem Cell 13:246 (2013)
- Geti I; Ormiston M: Touhain F; Toshner M; Movassagh M; Nichols J; Mansfield W; Southwood M; Bradley A; Rana AA; Vallier L; Morrell NW. ” practical and efficient cellular substrate for the generation of induced pluripotent stem cells. Stem Cells Trans Med 1:855 (2012)
- Yoshida Y; Takahashi K; Ishisaka T; Yamanaka S. Hypoxia Enhances the Generation of Induced Pluripotent Stem Cells. Cell Stem Cell 5:237 (2009)
Additional Publications
- Danisovic L; Csobonyeiova M; Nicodemou A; Novakova ZV; Miko M; Zamborsky R; Varga I. Generation and characterization of human iPSCs from human fibroblasts in respect to osteochondral regeneration. FASEB J 33:Ib168 (2019).
- Goa X; Yourick JJ; Sprando RL. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel. Stem Cell Research https://doi.org/10.1016/j.scr.2018.07.013: (2018)
- Goa X; Yourick JJ; Sprando RI. Comparative transcriptomic analysis of endothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cells. Stem Cell Research 25:202-212 (2017)
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