QA-Bio Deglycosylation kits 糖基化試劑套組
Deglycosylation kits 糖基化試劑套組 ，PNGase F 通常用來去除只包含 N-linked 醣鏈醣基化之醣蛋白，不管是變性和 native 條件之下都可以使用。Native 的樣品若是緩慢被 PNGase F 切割或者經過 PNGase F 切割後的析出物，這兩種情況下建議使用 Endo F Multi-Kit。兩種方式都可以使醣鏈完整並且可進行後續分析使用。
如果醣鏈的型式是未知的，或者同時具有 N-linked 和 O-linked 型式的醣鏈，建議使用 Enzymatic CarboRelease Kit 或者 Enzymatic DeGlycoMx Kit。這些 kits 包含一些酵素可以將醣鏈分解為單醣。DeglycoMx Kit，酵素包裝成預先混合好的形式存放在 20 microlitre 管中。CarboRelease Kit，酵素分開存放在獨自 20 microlitres 管中，使實驗設計及使用起來更有彈性。
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品牌：QA-Bio
PNGase F 通常用來去除只包含 N-linked 醣鏈醣基化之醣蛋白，不管是變性和 native 條件之下都可以使用。Native 的樣品若是緩慢被 PNGase F 切割或者經過 PNGase F 切割後的析出物，這兩種情況下建議使用 Endo F Multi-Kit。兩種方式都可以使醣鏈完整並且可進行後續分析使用。
如果醣鏈的型式是未知的，或者同時具有 N-linked 和 O-linked 型式的醣鏈，建議使用 Enzymatic CarboRelease Kit 或者 Enzymatic DeGlycoMx Kit。這些 kits 包含一些酵素可以將醣鏈分解為單醣。DeglycoMx Kit，酵素包裝成預先混合好的形式存放在 20 microlitre 管中。CarboRelease Kit，酵素分開存放在獨自 20 microlitres 管中，使實驗設計及使用起來更有彈性。
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| 產品貨號 | 產品名稱 | 描述 | 產品規格 (Datasheet) |
|---|---|---|---|
| KE-DGMX | Enzymatic DeGlycoMx Kit | The Enzymatic DeGlycoMx™ Kit will remove all N-linked oligosaccharides and many O-linked oligosaccharides from glycoproteins. N-links (Asnlinked) are removed using the enzyme PNGase F. In addition, all Ser/Thr-linked (O-linked) Gal-(β1-3)-GalNAc-(α1) and all sialic acid substituted Gal-(β1-3)-GalNAc-(α1) will be removed using the combinationof Sialidase and O-Glycosidase. The addition of ß-Galactosidase and Glucosaminidase will assist in the deglycosylation of larger O-link structures. | |
| KE-DG01 | Enzymatic CarboRelease Kit | The Enzymatic CarboRelease Kit will remove all N-linked oligosaccharides and many O-linked oligosaccharides from glycoproteins. N-links (Asn-linked) are removed using the enzyme PNGase F. In addition, all Ser/Thrlinked (O-linked) Gal-(β1-3)-GalNAc-(α1) and all sialic acid substituted Gal-(β1-3)-GalNAc-(α1) will be removed using the combination of Sialidase and O-Glycosidase. The addition of ß-Galactosidase and Hexosaminidase will assist in the deglycosylation of larger O-link structures. | |
| KE-EFX3 | Endo F Multi-Kit | QA-Bio™ Endo F1 cleaves Asparagine-linked (N-linked) high mannose or hybrid oligosaccharides. Endo F2 cleaves N-linked biantennary oligosaccharides and high mannose (at a 40X reduced rate).Endo F3 cleaves free or N-linked fucosylated biantennary or triantennary oligosaccharides,as well as triamannosylchitobiose core structures.These enzymes cleave between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine.The recombinant version is not glycosylated, which may result in properties differing from the native protein. | |
| E-PNG01 | PNGase F -0.3U/60ul | QA-Bio™ PNGase F cleaves asparagine-linked (N-linked) oligosaccharides from glycoproteins. PNGase F deaminates asparagine to aspartic acid, leaving the oligosaccharides intact. Denaturation increases the rate of cleavage up to 100x. Most native proteins can still be completely N-deglycosylated but incubation time must be increased. PNGase F will remain active under incubation conditions for at least 72 hours. PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins; for this purpose, use peptide N-glycosidase A. |
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