產品特點
使用 NutriStem hPSC XF Medium 培養 iPS 細胞至 20 代，細胞表型行程密集的單層細胞，有明顯的細胞群邊緣。
| A. Oct4 | B. DAPI | C. Merge |
| D. SSEA-4 | E. DAPI | F. Merge |
使用 NutriStem Medium 培養細胞 5 代之後，幹細胞特徵螢光表現。
從 24 小時開始記錄培養 6 代的幹細胞生長速率， NutriStem Medium 與 mTeSR1 的細胞生長效率相似。
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| 產品貨號 | 產品名稱 | 產品容量 |
|---|---|---|
| 01-0005 | NutriStem hPSC XF Medium for Human iPS and ES Cells | 100mL |
| 01-0005-100 | NutriStem hPSC XF Medium for Human iPS and ES Cells | 500mL |
Additional Publications
- Shimada M; Tsukada K; Kagawa N; Matsumoto Y. Reprogramming and differentiation-dependent transcriptional alteration of DNA damage response and apoptosis genes in human induced pluripotent stem cells. J Radiation Res 2019:1-10 (2019).
- Brodaczewska KK; Nielcka ZF; Maliszewska-Olejniczak K; Szxzylik C; Porta C; Bartnik W; Czarnecka AM. Metastatic renal cell carcinoma cells growing in 3D on poly‑D‑lysine or laminin present a stem‑like phenotype and drug resistance. Oncology Reports in press:2402 (2019).
- Bredenkamp N; Yang J; Clarke J; Stirparo GG; von Meyenn F; Baker D; Drummond R; Li D; Wu C; Rostovskaya M ;Smith A; Guo G. Efficient RNA-mediated reprogramming of human somatic cells to naïve pluripotency facilitated by tankyrase inhibition. bioRxiv https://doi.orb/10.1101/636670: (2019).
- Nakajima M; Yoshimatsu S; Sato T; Nakamura M; Okahara J; Sasaki E; Shiozawa S; Okano H. Establishment of induced pluripotent stem cells from common marmoset fibroblasts by RNA-based reprogramming. Biochem Biophys Research Commun in press:https://doi.org/10.1016/j.bbrc.2019.05.175 (2019).
- Liu L-P; Li Y-M; Guo N-N; LI S; Ma X; Zhang Y-X; Gao Y; Huang J-L; Zheng D-X; Wang L-Y; Xu H; Hui L; Zheng Y-W. Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation. Cell Reports 27:455-466.e5 (2019).
- Sacco AM; Belviso I; Romano V; Carfora A; Schonauer F; Nurzynska D; Montagnani S; Di Meglio F; Castaldo C. Diversity of dermal fibroblasts as major determinant of variability in cell reprogramming. J Cell Mol Med :1-13; https://doi.org/10.1111/jcmm.14316 (2019).
- Nishishita N; Muramatsu M; Kawamata S. An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions. A J Stem Cells 4:38 (2015).
- Klein T; Klug K; Henkel L; Kwok CK; Edenhofer F; Klopocki E; Kurth I; Üceyler N. Generation of two induced pluripotent stem cell lines from skin fibroblasts of sisters carrying a c.1094C>A variation in the SCN10A gene potentially associated with small fiber neuropathy. Stem Cell Res 35:101396 (2019).
- Supharattanasitthi W; Carlsson E; Sharif U; Paraoan L. CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection. Sci Rep 9:174 (2019).
- Su S; Guntur AR; Nguyen DC; Fakory SS; Doucette CC; Leech C; Lotana H; Kelley M; Kohil J; Martino J; Sims-Lucas S; Liaw L; Vary C; Rosen CJ; Brown AC. A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome. Cell Reports 25:3215-3228.e9 (2018).
- Ma L; Hu J; Cao Y; Xie Y; Wang H; Fan Z; Zhang C; Wang J; Wu C-T; Wang S;. Maintained Properties of Aged Dental Pulp Stem Cells for Superior Periodontal Tissue Regeneration. Aging and Disease :https://doi.org/10.14336/AD.2018.0729 (2018).
- Goa X; Yourick JJ; Sprando RL. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel. Stem Cell Researchhttps://doi.org/10.1016/j.scr.2018.07.013: (2018)
- Goa X; Yourick JJ; Sprando RI. Comparative transcriptomic analysis ofendothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cells. Stem Cell Research25:202-212 (2017)
- Rajasingh S; Thangavel J; Czirok A; Samanta S. “Generation of functional cardiomyocytes from efficiently generated human iPSCs and a novel method for measuring contractility.” PLoS one (2015)
- Poleganov MA; Eminli S; Beissert T; Herz S; Moon J-I; Goldmann J; Beyer A; Heck R; Burkhart I; Roldan DB; Tureci O; Yi K; Hamilton B; Sahin U. Efficient reprogramming of human fibroblasts and blood-derived endothelial progenitor cells using nonmodified RNA for reprogramming and immune evasion. Human Gene Therapy 26:751 (2015)
- Durruthy JD; Sebastiano V. Derivaton of GMP-compliant integration-free hiPSCs using modified mRNAs. Methods Mol Biol 1283: 31 (2014)
- Greber, B., Coulon, P., Zhang, M., Moritz, S., Frank, S., Müller-Molina, A.J., Araúzo-Bravo, M.J., Han, D.W., Pape, H.C., and Schöler, H.R. FGF signalling inhibits neural induction in human embryonic stem cells. EMBO J 30: 4874-4884(2011).
- Sugii, S., Kida, Y., Berggren, W.T., and Evans, R.M. Feeder-dependent and feeder-independent iPS cell derivation from human and mouse adipose stem cells. Nat Protoc 6(3): 346-358 (2011).
- Warren, L., Manos, P.D., Ahfeldt, T., Loh, Y.H., Li, H., Lau, F., Ebina, W., Mandal, P.K., Smith, Z.D., Meissner, A., Daley, D.Q., Brack, A.S., Collins, J.J., Cowan, C., Schlaeger, T.M., and Rossi, D.J. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7: 618-630 (2010).
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