RS09 imatrix 511 300
RS09 imatrix 511 300

iMatrix-511

iMatrix-511 適用於其他無餵養細胞的細胞培養液 (feeder-free media)，使用不同無餵養細胞的細胞液，皆適用 iMatrix 來成功培養 iPSC。廣泛應用在幹細胞培養的相關研究，例如建立 iPSC，適用於重新編程 iPS 細胞的過程，每組批號的產品皆經過 iPSC 培養測試。

brands logo Reprocell

分類：其他試劑 > 幹細胞相關產品 > 試劑溶液

標籤：

品牌：ReproCELL

原廠介紹

無餵養系統 (Feeder free) 培養 hESC /iPSC 最佳塗佈液！！！

  • 適用於其他無餵養細胞的細胞培養液 (feeder-free media)

  • 廣泛應用在幹細胞培養的相關研究，例如建立 iPSC

  • 適用於重新編程 iPS 細胞的過程

  • 每組批號的產品皆經過 iPSC 培養測試

使用方式

laminin 511 pic01

STEP1 Coating

  • 以 PBS 稀釋 iMatrix-511
  • 以 05 ug/cm2 塗佈細胞培養盤

STEP2 Seeding

  • 移除多餘的塗佈液
  • 直接將細胞培養在未乾燥的培養盤上

培養實例

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A) FibroblastsB) EPCsC) UDCs

由不同種類細胞重新編程為 iPSC 於 iMatrix 上培養。使用螢光染劑標定幹細胞 (Stemgent StainAlive TRA-1-60 Antibody, Cat. No.09-0068)

A) NutriStemB) StemFit

使用不同無餵養細胞的細胞液，皆適用 iMatrix 來成功培養 iPSC

Stem Cell Culture Substrate​

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產品貨號產品名稱產品描述產品容量
NP892-011iMatrix-511highly purified and refined laminin-511 E8 fragments, produced in CHO cells175 ug × 2 tube/box
NP892-012175 ug × 6 tube/box
NP892-013175 ug × 1 tube/box
NP892-021iMatrix-511 SILKxeno-free, recombinant laminin-511 E8 fragments, produced in recombinant silkworm cocoon​175 ug × 6 tube/box​
NP892-022175 ug × 1 tube/box​

Endothelial Cell Substrate​

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產品貨號產品名稱產品描述產品容量
NP892-041iMatrix 411highly purified and refined product of human recombinant laminin-411 (E8 fragment) expressed by CHO-S cells.175 ug × 2 tube/box
NP892-042175 ug × 6 tube/box
NP892-043175 ug × 1 tube/box

Cardiac and Myoblast Cell Culture Substrate​​

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產品貨號產品名稱產品描述產品容量
NP892-061iMatrix 211highly purified and refined product of human recombinant laminin-221 (E8 fragment) expressed by CHO-S cells. ​175 ug × 2 tube/box
NP892-062175 ug × 6 tube/box
NP892-063175 ug × 1 tube/box

  1. Takayama K. et al., “Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells”. Biochemical and Biophysical Research Commun.474 (1): 91-96 (2016).
  2. Nishimura K. et al., “Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1”. Stem Cell Reports6  (4): 511-524 (2016).
  3. Matsuno K. et al., “Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells”. Differentiation2016.04.002.
  4. Hayashi R. et al., “Co-ordinated ocular development ofrom human iPS cells and recovery of corneal function”. Nature531, 368-80 (2016),
  5. Sasaki K. et al., “Robust in vitro induction of human germ cell fate from pluripotent stem cells”. Cell Stem Cell 17 (2):178-194 (2015).
  6. Okumura N. et al., “Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells”. Invest Ophthalmal Vis Sci.56 (5), 2933-42 (2015).
  7. Nakagawa M. et al., “A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells”. Scientific Reports 4: 3594 (2014).
  8. Miyazaki T. et al. “Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells.” Nature Communications 3: 1236 (2012).
  9. Taniguchi Y. et al., “The C-terminal region of laminin β-chains modulates the integrin-binding affinities of laminins.” J. Biol. Chem.284 (12): 7820-31 (2009).
  10. Ido H. et al., “The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by laminins.” J. Biol. Chem.282 (15): 11144-54 (2017).

Additional Publications